Composite

Part:BBa_K3182108:Design

Designed by: Oliver Hild Walett   Group: iGEM19_Linkoping_Sweden   (2019-07-14)


pT7-CBDcipA-sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 580
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 598


Design Notes

Used this great table to find a suitable restriction enzyme that would fit inside the thrombin site. BamHI was chosen cause it codes for GS, which is also the end of the thrombin site. This enables the C-terminal fusion protein of the CBDcipA to be changed.

Source

Expression system: T7 bacteriophage
CBDcipA: Clostridium thermocellum
sfGFP: Aequorea victoria (heavily mutated)

References